The subfamily is monophyletic (Grimali 1990; Sidorenko 2002), but can not be characterised by a single morphological character that distinguishes this subfamily from the Drosophilinae. See for a discussion on this subfamily "Drosophila: A Laboratory Handbook" by M. Ashburner, S. Hawley, K. Golic (not reproduced here due to copyrights).
- D. A. Grimaldi 1990. A phylogenetic, revised classification of genera in the Drosophilidae (Diptera). Bulletin of the American Museum of Natural History 197: 1-128.
- V. S. Sidorenko 2002. Phylogeny of the tribe Steganini Hendel and some related taxa (Diptera, Drosophilidae). Far Eastern Entomologist 111: 1-20.
The phylogenetic relationships within the subfamily have not yet been confirmed by molecular studies, and are partially based on traditional cladistic methods using primarily morphological characteristics and partly based on expert opinions. The classification below might be not approved by all scholars in the field, but gives a reasonable overview of our current knowledge:
- Subtribe: Gitonina
- Subtribe: Acletoxenina
- Subtribe unknown
For species within the various genera, see Taxodros